If you have found that a zipper slider is not moving up and down or will not close any of the zipper teeth, it may be because the zipper has become unaligned. Over time, zipper teeth will not match up or become stuck, causing accessories such as backpacks and purses to become almost unusable. This guide will teach you how to re-align a zipper on a backpack with a needle, thread, and pliers. Although some background knowledge in basic sewing skills is recommended, we will briefly explain some sewing techniques. We will be show you how to fix a backpack that will not zip properly by re-aligning an uneven zipper without the use of a sewing machine.
Citation: Jiang Y, Giase G, Grennan K, Shieh AW, Xia Y, Han L, et al. (2020) DRAMS: A tool to detect and re-align mixed-up samples for integrative studies of multi-omics data. PLoS Comput Biol 16(4): e1007522.
Investigation of complex traits and disorders can use multiple omics data to systematically explore regulatory networks and causal relationships. Sample mix-ups can occur in omics experiments during sample collection, handling, genotyping, and data management. As the number of datasets to be integrated increases, the likelihood of error also multiplies. Sample mix-ups reduce statistical power and generate false findings. Not only is the detection and re-alignment of errors in data identifications (IDs) critical to ensuring accurate findings in integrative studies, such corrections can increase statistical power thus the number of positive findings .
For multi-omics data, the sample re-alignment procedure can be generally divided into two steps: first, to estimate genetic relatedness among the data of different omics and group together all the data of the same individual; then, to assign potential IDs for each data group. It is well-known that genetic information from the same individual should be identical regardless of the omics from which it originated. Using genotype data as a mediator, data originated from the same individual can be grouped together.
Several tools have been developed to estimate genetic relatedness for multi-omics data in many different ways, such as genotype concordance [2, 3], correlation of different quantifications [1, 2], correlation of variant allele fractions , concordance of sequencing reads , etc. The various methods made it possible to compare different data types, such as DNA sequencing, RNA sequencing, SNP array, etc. However, these tools mainly focused on implementing the first step of sample re-alignment, that is, to estimate genetic relatedness among multi-omics data. None of the existing tools has a systematic solution on determining the potential IDs for each data.
Here, we described DRAMS, a tool to Detect and Re-Align Mixed-up Samples that leverages sample relationships in multi-omics data by directly comparing genotype data. DRAMS also uses a logistic regression model followed by a modified topological sorting algorithm to systematically re-align misclassifications. This tool integrates sample relationships among different omics types, concordance rates of genetics-based sexes and reported sexes, etc. With this design, DRAMS can be applied to as many omics types as possible. Using both simulated data and real data, we proved the accuracy and power of DRAMS in studies involving multi-omics data.
The goal of DRAMS is to detect and re-align mix-ups based on the grounds that all omics data originating from the same individual should match genotypes. DRAMS operates on a two-step process: first, we ensure that all omics data of the same samples were grouped together by their genotypic relatedness; after that, we find out the potential true ID of each data group (Fig 1).
I have a pdf (NOT the original document). At the top of each page is a headline that's centered. My client wants me to add on a few words to these headlines. However, when I do this, the text just adds to the right. I can't see where to re-align the text so it's once again centered. Or at least get them all left-justified. Any ideas?
Aligning and re-aligning a team is a never-ending process. When you take time to align team regularly, it will be easier to prevent conflicts. Consider doing an alignment check quarterly, so your team can quickly make adjustments to ensure they are performing well and happy.
Dignity Health, Yavapai Regional Medical Center (YRMC) has re-aligned its Prescott outpatient laboratory draw centers into a single, state-of-the-art location. The new Outpatient Laboratory Draw Center is now serving patients in the recently opened Outpatient Services Building (OSB) West, on the campus of YRMC West at: 041b061a72